PCR vs DNA Sequencing

Similarities

·      Both techniques use DNA polymerase and primers for elongation.

·      Gel electrophoresis is used to separate the different lengths of DNA produced at the end of both processes.

PCR

·         Utilizes a special polymerase called taq polymerase which can withstand high temperature.

·         Instead of RNA primer, DNA primers are use to initiate the elongation.

·         Heating is used to break the hydrogen bonds between the nitrogen bases, forming single stranded DNA.

DNA Sequencing

·      Dideoxynucleotides cause the new strand of DNA to terminate at various points due to the absence of hydroxyl group attached to the 3’ carbon.